FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30226067
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC50800.012.356111
TCAGGTA65050.010.0651367
TAGAACA64450.09.77408312
GGTAGAA63950.09.639420510
CAGGTAG67550.09.1924768
GGAAAAT128800.09.0560911
GTACTCG41500.08.8982364
AATTCAC47700.08.401585
AATCGAA63100.08.3135385
AAAAATC86750.08.3062962
AGAGATC132800.08.189852
AGATCAG112900.08.0374694
CGCGAAT33800.07.8612611
AAAATCC93900.07.8415883
GAAAAAT183000.07.8258751
ATTCACC47550.07.7607296
TTACGAC39900.07.66856529
AACGGAC19700.07.54287675
CGTTAAC48700.07.530565721
GGGGAAT64750.07.51174551
Produced by FastQC (version 0.11.5)