FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27407853
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC45250.011.18681411
TCAGGTA62000.09.2538397
TAGAACA56500.09.0389912
GGTAGAA59350.08.87028710
GGAAAAT132250.08.7687511
GTACTCG44100.08.6797814
CAGGTAG63850.08.3861488
AATTCAC45700.08.3750995
GAATTGA65600.08.3063454
AAAATCC94350.08.2813943
ACATGCG36850.07.9380856
CGTGACT19500.07.8523232
AAAAATC85450.07.8001592
GGAGAAT90100.07.52259871
ATTAGGG25500.07.5004736
AATTGAC48500.07.42737675
GAATCGA40950.07.42298034
AATCGAA52500.07.41897735
ACTTTCG17300.07.413126519
CGGAAAT24350.07.39804941
Produced by FastQC (version 0.11.5)