FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28115490
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG24400.012.1709716
GTAGAAC41450.012.04964311
TCGTGTA30100.010.23997815
GAGTGTA29900.010.15711728
GTAGATC31650.010.09411132
TGTAGGG31300.09.84779418
AGTGTAG33550.09.4553729
TAGAACA53550.09.41096712
TCAGGTA60750.09.2961547
GTACTCG43400.09.2349344
GTGTAGA36550.09.23330930
ACATGCG34400.08.8304666
GGTAGAA58600.08.79191710
TCTCGGT37400.08.60187637
GGAAAAT131900.08.4848111
CGCGATA16650.08.37778829
GTAGGGA39550.08.30563719
ACTTTCG22450.08.21796119
CAGGTAG64650.08.1432638
AATCGAA58350.08.1028235
Produced by FastQC (version 0.11.5)