FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25494301
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG39550.024.34636916
GAGTGTA43350.022.26291828
AGTGTAG44400.021.33272429
TGTAGGG45950.021.10352918
TCGTGTA46100.020.78973815
GTAGATC46900.020.62744132
GTGTAGA49100.019.84058230
GTGTAGG49600.019.45814717
GTAGGGA52850.018.60371819
TAGGGAA56750.017.52345720
TCTCGGT55100.017.39371737
TAGATCT56700.017.14156233
ATCTCGG57850.016.91694636
ATCGGAA63400.016.373533
GAGCGTC65750.015.50042610
AGAGTGT66450.014.92971727
TCGGAAG68000.014.7355224
GTCGTGT67900.014.512609514
GATCGGA75450.014.2063822
AGAGCGT71800.014.162979
Produced by FastQC (version 0.11.5)