FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29504452
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC50300.011.04760711
GGAAAAT153100.09.5017221
TCAGGTA65000.09.1382187
TAGAACA63250.08.96353412
GGTAGAA68800.08.4039610
GAATCGA43600.08.3149174
GTACTCG46150.08.2458134
AATTGAC51150.08.2312655
AATCGAA61600.08.1506975
GAATTGA75450.08.1474544
CGTGACT18150.08.0644982
CAGGTAG72950.08.0493428
AAAATCC100700.07.93842463
AAATCCT101450.07.679674
ACTTTCG18200.07.66451319
TTACGAC32050.07.65144829
CTCGAAT26600.07.61913871
TCGGGGG79600.07.46118939
ATCTAAC19150.07.40255646
ATCGAAC33100.07.3418536
Produced by FastQC (version 0.11.5)