FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n02_S1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n02_S1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27338506
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25750.011.3684922
GTAGAAC30600.09.70488311
GTACTCG56450.09.4536874
GCGTGAC34600.09.4370221
GGAAAAT116200.09.0113511
AATTGAC48000.09.0059085
TACTCGA59150.08.983075
GAATTGA70800.08.8733534
AATCGAA49500.08.7330025
AGTACTC63750.08.6893263
CTCGAAT22600.08.6686981
ATTGACC46250.08.0761876
AAAATCC88700.08.0222223
CGGAAAT23700.07.8862911
ACACGCG28950.07.69478856
AAATTGA78150.07.6066214
GTGACTC41150.07.60634663
GGGAAAT55750.07.59374571
GAATCGA39450.07.53431464
ATCTAAC15100.07.45080336
Produced by FastQC (version 0.11.5)