FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13111639
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG6250.035.17473615
CGTCTGA6450.034.08404516
ACACGTC7050.032.12744513
CACGTCT6950.031.95558414
CACACGT7250.030.61938312
GCACACG9150.024.50632511
AGCACAC9300.024.1110610
TCGGAAG10850.023.7757743
ATCGGAA10800.023.6781442
CAGTCAC6300.023.6060227
GATCGGA11450.022.534611
CCAGTCA7850.022.40177326
ACCGATC2450.022.10068534
CCGAGAC3100.021.82496529
CTCCAGT9700.021.36751224
CGGAAGA12200.021.3287144
AACTCCA10500.020.81159222
GAGACCG3300.019.13865531
AGAGCAC12300.018.4126178
TTACGGG750.001136814517.938638
Produced by FastQC (version 0.11.5)