FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33897302
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG60150.027.79049515
CGTAATC59100.026.95497138
CGTCTGA61750.026.88824816
TCGTATG59050.026.6384745
CTCGTAT61550.025.48298644
CCGTACG64700.024.96785233
TCGGAAG77750.021.5933763
ATCGGAA78850.021.0923442
TACGTAA77900.020.85231836
ACGTAAT80550.020.05534737
GTAATCT81750.019.87336239
CACGTCT87050.019.69361514
CACACGT93400.018.20980312
CAGTCAC96200.017.54102127
ACCGTAC102600.017.47715432
ACACGTC98950.017.4385313
CACCGTA94850.017.31590831
AGTCACC101400.016.86324728
TAATCTC98950.016.60153240
GATCGGA109800.015.3553251
Produced by FastQC (version 0.11.5)