FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28429907
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG534260.18792182471789304TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG83900.037.6236733
AGTCACG88950.035.48720628
ACGTCTG92800.034.8619715
CGTCTGA94850.034.32196816
TCGTATG82350.033.6945545
TTTCGGA93900.033.32912435
CTCGTAT84250.032.8540244
TTCGGAA95150.032.67777636
ACGTTTC97800.032.46026632
GTTTCGG98800.032.04063434
ATCGGAA103900.031.3205172
GTCACGT104450.030.32937429
TCGGAAG109000.029.9376493
TCGGAAT106750.028.89632637
CACACGT116600.027.95736912
CACGTTT115750.027.4264731
CACGTCT122100.026.90136714
ACACGTC123650.026.59988413
CAGTCAC123150.026.28988827
CGGAATC119100.025.37245638
Produced by FastQC (version 0.11.5)