FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23105928
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG769900.3332045352171097TruSeq Adapter, Index 20 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT457340.19793189003272232TruSeq Adapter, Index 20 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319850.13842767968462466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG103400.035.19248245
AGATCGG90300.026.2487831
ACGTCTG158000.024.44893315
AGTCACG156700.024.2653228
CGTCTGA160800.023.98123216
CACGTGG159850.023.87082531
CTCGTAT156850.023.3858544
GTCACGT166550.022.88422229
TCGGAAG170100.022.6902873
CACGTCT171250.022.6620214
ATCGGAA170300.022.610782
ACGTGGC170150.022.45225332
GCCTTAT168700.022.33941737
TATCTCG171600.021.80808441
CAGTCAC179900.021.4239827
GGCCTTA178600.021.30166636
ACACGTC183550.021.16727413
CACACGT183550.021.13050512
TCACGTG184400.020.72939730
CGGAAGA188900.020.5988624
Produced by FastQC (version 0.11.5)