FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30226067
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG313360.10367210527257814TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG58700.030.82267845
ACGTCTG60800.030.49071115
AGTCACG59100.030.45570828
CGTGAAA64250.028.11882233
CGTCTGA66150.027.9907116
CTCGTAT56950.027.62068444
ACGTGAA69850.025.80002232
GTCACGT71350.025.25817129
TGAAACG70650.025.09401735
GAAACGA71700.024.94585236
TCGGAAG74800.024.9121173
ATCGGAA74750.024.8083522
CACGTGA74850.024.28698231
AACGATC65100.023.88321138
AAACGAT76200.023.17856837
CACGTCT87550.021.91959414
CACACGT88650.021.21568712
TCACGTG89500.020.48754930
ACACGTC98450.019.72080413
CAGTCAC95950.019.20478227
Produced by FastQC (version 0.11.5)