FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27407853
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG49100.027.35645928
ACGTCTG51650.026.70136315
CGTCTGA53400.025.78418716
TCGTATG52750.025.21306245
CTCGTAT52950.024.86227844
ACGTCCG56000.023.90500532
GTCCGCA59750.022.59304834
GTCACGT61100.022.35190429
ATCGGAA65050.021.2768142
TCGGAAG68300.020.5938763
CCGCACA69200.019.18224736
CACACGT73450.019.08212912
CACGTCT74700.018.8535114
TCACGTC72150.018.74122830
CGCACAT73650.017.90186537
ACACGTC84300.016.81293513
CAGTCAC85050.016.50734927
TCCGCAC85200.016.0026935
TCTCGTA85850.015.30818143
GATCGGA93450.014.9096611
Produced by FastQC (version 0.11.5)