FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28115490
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG334400.11893799467837837TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG63050.027.79714215
TCGTATG60150.027.60996445
CGTCTGA66150.026.56250216
CTCGTAT64950.025.36171544
GTCCGCA70350.024.33711636
ATCGGAA80300.021.8599382
TCGGAAG81750.021.7199613
CACGTCT86950.020.6219814
CACACGT89050.020.1855512
ACCCGTC91200.019.21769132
CGCATCT88400.019.01500539
ACACGTC95900.019.00177213
CAGTCAC97650.018.17936927
CCGTCCG100900.017.6154534
AGTCACC105350.017.23494728
TCTCGTA97650.016.91494243
GATCGGA106050.016.704861
TCCGCAT102150.016.6514837
CCCGTCC106500.016.54134233
TCACCCG106250.016.4108930
Produced by FastQC (version 0.11.5)