FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25494301
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG267710.10500778193526467TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG49550.025.84132245
ACGTCTG76300.021.79058615
CGTCTGA79150.020.9491116
CTCGTAT69600.018.23535744
TCGGAAG92700.018.2323993
ATCGGAA92000.018.1998882
CACACGT99600.016.87283712
CACGTCT101100.016.75673714
ACACGTC106000.015.93899413
GTCAGAA106700.015.47678236
TGTCAGA108300.015.37310835
GATCGGA109200.015.29466151
CAGTCAC110450.015.15572427
GTCACAT111200.014.99298129
AATCTCG101400.014.64685741
AGTCACA113400.014.64250328
AGATCGG65850.014.4249821
ATGTCAG124650.013.55530934
TCTCGTA97050.013.40221943
GAGCACA130400.013.3360989
Produced by FastQC (version 0.11.5)