FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29504452
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG714130.2420414383564894TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG103600.034.23304445
TCCGTAT109250.033.87713237
CCGTATC109150.033.6419138
CGTATCT109100.033.39166339
AGTTCCG112800.033.32933434
CTCGTAT109150.032.67704444
ACGTCTG119400.032.16443315
TTCCGTA119500.030.87653536
CGTCTGA124250.030.87270716
GTTCCGT130100.028.568835
TATCTCG129250.028.0302841
ATCGGAA137700.027.8826872
TCGGAAG140550.027.4293843
GTCACAG140250.027.06313329
CACACGT145550.026.52392612
CACGTCT146700.026.31653814
TCTCGTA142100.025.47998843
TCACAGT148900.025.46057130
CAGTCAC152900.025.25108527
ACACGTC158400.024.62786113
Produced by FastQC (version 0.11.5)