FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l08_n01_S1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l08_n01_S1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27338506
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA289280.10581412166414653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG45200.027.87407715
CGTCTGA47300.026.82680316
TCGTATG45100.026.2948145
CTCGTAT48750.023.07976244
TCGGAAG60300.021.3112033
ATCGGAA61500.020.7124022
CACGTCT63350.020.42058614
CACAGTC64050.019.21409431
CACACGT69150.018.90231712
GTCACAG68700.017.979729
CAGTCAC73900.017.1713627
AGTCACA75950.016.50039128
TCACAGT76950.016.13923630
ACACGTC83150.016.09851313
AATCTCG76450.015.42354441
ACAGTCA83950.015.38306732
GATCGGA88200.014.5217421
AGCACAC94050.014.42412910
AGTCAAC87200.014.29376434
GAGCACA98200.013.9978339
Produced by FastQC (version 0.11.5)