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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-04-03, 20:04 based on data in: /beegfs/mk5636/logs/html/CBKDUACXX/7

        General Statistics

        Showing 22/22 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CBKDUACXX_l07_n01_C1W1
        60.1%
        52%
        11.1
        CBKDUACXX_l07_n01_C1W2
        60.5%
        53%
        11.0
        CBKDUACXX_l07_n01_C1W3
        58.5%
        52%
        10.6
        CBKDUACXX_l07_n01_C1W4
        58.0%
        53%
        11.7
        CBKDUACXX_l07_n01_C1W5
        59.5%
        52%
        12.5
        CBKDUACXX_l07_n01_C1W6
        59.1%
        52%
        10.7
        CBKDUACXX_l07_n01_C1W7
        57.9%
        51%
        9.9
        CBKDUACXX_l07_n01_C1W8a
        53.3%
        52%
        6.7
        CBKDUACXX_l07_n01_C1W8b
        52.9%
        52%
        6.1
        CBKDUACXX_l07_n01_C1W9
        59.2%
        52%
        10.1
        CBKDUACXX_l07_n01_undetermined
        90.3%
        45%
        5.4
        CBKDUACXX_l07_n02_C1W1
        64.2%
        54%
        11.1
        CBKDUACXX_l07_n02_C1W2
        64.8%
        54%
        11.0
        CBKDUACXX_l07_n02_C1W3
        62.5%
        54%
        10.6
        CBKDUACXX_l07_n02_C1W4
        62.2%
        55%
        11.7
        CBKDUACXX_l07_n02_C1W5
        63.9%
        54%
        12.5
        CBKDUACXX_l07_n02_C1W6
        63.2%
        54%
        10.7
        CBKDUACXX_l07_n02_C1W7
        61.4%
        54%
        9.9
        CBKDUACXX_l07_n02_C1W8a
        55.7%
        53%
        6.7
        CBKDUACXX_l07_n02_C1W8b
        54.9%
        53%
        6.1
        CBKDUACXX_l07_n02_C1W9
        63.0%
        54%
        10.1
        CBKDUACXX_l07_n02_undetermined
        90.3%
        45%
        5.4

        Lane 7 Demultiplexing Report

        Lane 7 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 11/11 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        5,414,739
        5.1
        C1W1
        11,116,642
        10.5
        C1W2
        11,037,281
        10.4
        C1W3
        10,644,350
        10.1
        C1W4
        11,722,313
        11.1
        C1W5
        12,455,249
        11.8
        C1W6
        10,658,714
        10.1
        C1W7
        9,947,134
        9.4
        C1W8a
        6,658,768
        6.3
        C1W8b
        6,149,939
        5.8
        C1W9
        10,086,141
        9.5

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        CCCCCC
        80996.0
        1.5
        CCCCTC
        42633.0
        0.8
        TCAGTT
        39466.0
        0.7
        TCCCTT
        36660.0
        0.7
        CCCCTT
        36372.0
        0.7
        TAAGTT
        34738.0
        0.6
        TCACTT
        34411.0
        0.6
        CCAGTT
        34294.0
        0.6
        CCACCC
        34245.0
        0.6
        CCACTT
        33667.0
        0.6
        CCCACC
        32257.0
        0.6
        CCAATT
        31596.0
        0.6
        TCAATT
        31060.0
        0.6
        CCCGCC
        28906.0
        0.5
        TCCCTC
        28524.0
        0.5
        TAACTT
        28428.0
        0.5
        CACCCC
        27912.0
        0.5
        TAAATT
        27090.0
        0.5
        CCAACC
        26821.0
        0.5
        TCCGTT
        26652.0
        0.5

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        7.0
        114,676,928
        105,891,270
        5.1
        2.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.