FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5414739
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAGT250.002108836835.9963877
CACTCTT1750.027.0224952
AGTGTAG1700.025.1459329
GTGTAGA1800.023.74893230
TAGATCT2050.021.95023333
TCTCGGT2050.021.94901537
GAGTGTA2050.020.85021828
TGTAGAT2200.019.43094431
TCGGTGG2450.019.28235439
GTAGATC2350.019.14807532
CTCGGTG2350.019.14701338
GATCTCG2500.017.99919135
AGATCTC2750.017.18104634
GCGGGAT800.001702018416.8896851
GTGACTG800.001712467216.8742431
CGGTTCG1205.1833777E-616.8739312
TCGTGTA2650.016.13105215
GATCCGC850.002557160615.8913255
GGGCCCG850.002569957615.87943845
TAGGGAA3050.015.49096420
Produced by FastQC (version 0.11.5)