FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10086141
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT8550.013.6963072
GCGTGAC13050.010.6994761
TATTCGC14350.09.8774197
GTTACGC3053.7671343E-69.5895527
GTAGAAC11750.09.57347811
CTCGAAT8550.09.2189891
GTACTCG18150.09.1806614
GCATAGG14250.09.15640341
AATCGTA4752.7685019E-99.0054985
GGAAAAT41900.08.9760191
GTGACTC14900.08.9172283
CACTCGT21750.08.68918735
GTAGGCG14250.08.68374330
TAGGCGG14100.08.61655731
AATCGAA18600.08.5939575
AGTTACG3155.7173005E-58.5712216
AGCCTAG8150.08.5572725
TTAGATC8750.08.4932152
CGTAGGC15350.08.35459929
TACTCGA19150.08.3471335
Produced by FastQC (version 0.11.5)