FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6149939
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG4050.028.88590815
ATCTCGG19350.021.51063235
TCTCGGG9000.020.99800536
CGTGTAG14800.019.60946315
ATTCGGG9400.018.42909837
TGTAGGG15850.018.16773417
TTCGGGG28850.017.46801238
CGGAAAG13850.017.393895
GGGTAGA11050.017.30689229
TCCGGGG17850.017.26726538
TTTCGGG12900.017.265837
GGTAGAT10450.016.7934830
AGATCTC25550.016.73112533
GGGTAGG7250.016.44644516
GTGTAGG17950.016.41878516
GTAGATC16750.016.2529831
TAGATCT17000.016.14631332
GTAGGGG9100.016.07016218
TTGGGGG32550.016.0345639
TCGGAAA11050.015.8946724
Produced by FastQC (version 0.11.5)