FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6658768
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG4550.030.1612615
ATCTCGG20300.020.28221735
GGGTAGA10450.017.86990529
CGTGTAG17850.017.77100215
TCTCGGG9700.017.39471436
GTAGATC18550.017.3441331
GGTAGAT10000.017.32411830
TGTAGGG18950.017.09508517
GGGTAGG8700.016.80822816
TCTCGGT15400.016.65374636
TCCGGGG19100.016.60785538
TTGGGGG32950.016.3859139
GTAGGGG10750.016.32401518
ATTCGGG10600.015.70556837
TAGATCT20350.015.69944732
TTCGGGG27550.015.67860438
GTGTAGG20300.015.62610916
GGGGCCG27550.015.43293245
TCGGAAA13100.015.2993834
TTTCGGG12650.014.93882837
Produced by FastQC (version 0.11.5)