FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9947134
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG4050.016.10944215
CGTGTAG14200.015.36789316
GAGTGTA15350.014.36268128
TGTAGGG16350.013.34750818
AGTGTAG16700.013.20275529
ATCGGAA24050.012.3602323
GTGTAGA19150.012.21854630
TCTCGGG9400.011.72750836
GTAGATC19150.011.63117532
TCGTGTA18600.011.61169915
TCTCGGT18700.011.54960537
GTGTAGG19350.011.39382417
GTAGAAC12700.010.45167311
GTAGGGA22400.010.3451719
ATCTCGG22400.010.24447336
TCGGAAG25900.09.9111674
CACTCGT18850.09.90658335
TAGATCT22550.09.777698533
GGAAAAT41100.09.698821
GATCGGA34050.09.5239112
Produced by FastQC (version 0.11.5)