FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10658714
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG2650.018.67718915
CGTGTAG8900.012.63905815
GTAGAAC14750.010.6767911
TGTAGGG10900.010.5259217
GGAAAAT45150.010.3256691
GTACTCG17450.010.0654084
ATCTCGG17550.09.61491235
AAAATCC33350.09.1837943
TCTCGGG11050.08.9582936
CTAGCGG8550.08.9460068
TCGTGTA10850.08.91606814
GTAGATC13550.08.80030531
CGTGACT7250.08.6976762
CTCGAAT9100.08.4147871
GGTAGAA19050.08.38490410
GAAAATC39400.08.2880992
TACTCGA20700.08.2662815
GTGTAGG13350.08.25751816
TAGAACA20000.08.21159612
GTAGGGA14450.08.0962618
Produced by FastQC (version 0.11.5)