FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12455249
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG23850.011.8959854
GTAGAAC16650.011.62052311
CGTGACT11650.010.8246852
TACTCGA28150.010.0772575
AGTACTC30050.09.5171933
GGTAGAA23200.08.72762210
TAGAACA23300.08.40049312
CTCGAAT11000.08.3937931
GCGTGAC16150.08.3665381
AATCGAA26800.08.316695
TCCGTTA11150.08.27292619
TACGCAC6807.2759576E-128.2710779
TCAGGTA24050.08.2322357
GGAAAAT52300.08.2242971
AGCCTAG12450.07.950831425
GAATCGA20900.07.86492874
GTCTGAC15800.07.83921671
AAAAATC34600.07.810132
AAAATCC40800.07.7823113
AATTGAC22850.07.68526035
Produced by FastQC (version 0.11.5)