FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11722313
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT9750.013.3961692
GTAGAAC13650.011.86707711
CGGGTAG3358.094503E-1011.41689515
GTACTCG23950.010.7178674
GCGTGAC14850.010.3121881
CTCGAAT10600.09.5603761
AGTACTC27700.09.3491383
TACTCGA26800.09.2407975
TCAGGTA20500.09.2189277
CGTGTAG10550.08.74329915
GGAAAAT49000.08.5024481
GGTAGAA19050.08.38508110
AAAAATC30750.08.275432
TAGGCGG14700.08.11173131
CTATAAG13400.08.0657634
TAGAACA20650.08.06223812
TATCGGG4202.9264047E-68.0406935
GTGACTC16250.08.0376323
TCGTAGG15150.08.01864428
CGGAAAT11350.07.93656351
Produced by FastQC (version 0.11.5)