FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10644350
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG8850.011.439507515
AATCGAA21150.010.4325035
GTACTCG20100.010.4186334
CGTGACT9850.010.2883352
GTAGAAC12800.09.84274211
TGTAGGG11600.09.50298117
GAATCGA15950.09.3176664
GGAAAAT46800.09.2392861
AGTACTC24300.09.174783
GCGGAAA28200.09.0242771
CACTCGT25100.08.96377435
CTCTATA24950.08.92627145
GTAGATC12950.08.86063931
AGCCTAG10450.08.82668225
GTGTAGG12100.08.73869316
TACTCGA23450.08.7371825
ACTCGTG26450.08.50574636
GCGTGAC13000.08.4885951
GAAAAAT51950.08.4100641
TCACACG10300.08.30012115
Produced by FastQC (version 0.11.5)