FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n02_C1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n02_C1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11037281
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAG4150.017.34785515
CGTGACT8250.012.8288412
GTAGAAC14700.011.32552411
ATTCGGG12000.010.686501537
ATCTCGG24000.010.12446735
TTCGGGG30400.09.99084338
TTTCGGG12850.09.97961237
CGTGTAG18050.09.97141415
GGGGGGC64350.09.89364243
GTACTCG24600.09.7937474
AAAGGGG48700.09.65441126
GGGGGAA59450.09.65024121
TGGGGGG39800.09.552608540
TCGGGGG36400.09.51795439
GTAGATC18900.09.40426731
TCTCGGG12300.09.32839736
GGGGGCC43250.09.25862144
GCGTGAC13150.08.9052211
TGTAGGG21200.08.8078617
AGTACTC29700.08.7951863
Produced by FastQC (version 0.11.5)