FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5414739
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA3750.036.53047616
CACACGT3900.035.69666712
CACGTCT3900.035.12545414
ACGTCTG3950.034.6808315
ACACGTC4450.031.2844313
CAGTCAC3500.030.2479627
CCAGTCA3750.029.44449626
GCACACG5100.027.73773211
CTCCAGT4750.026.56473724
GATCGGA7300.026.1611821
ATCGGAA7350.025.9764962
AACTCCA5050.025.87901522
AGCACAC5500.025.72044210
TCGGAAG7400.025.497443
GGACTAT450.001239284224.9578113
CGGAAGA7850.024.0358034
TACACGA1152.6011548E-1023.51662644
AGAGCAC6450.021.5840328
CTGAACT6450.021.26968419
TGAACTC6700.020.51303520
Produced by FastQC (version 0.11.5)