FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10086141
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG152050.1507514122596541TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26400.030.51430745
CGTCTGA27150.030.32810216
ACGTCTG27450.030.32448215
AGTCACG26650.030.14093228
CTCGTAT27600.029.02540444
GTGGATA27800.028.72894936
TCGGAAG31200.026.256443
TATCTCG31250.025.70724541
CACGTCT33000.025.42897614
ATCGGAA32050.025.4196512
GTCACGA33350.024.15297929
GGATATC34100.023.7532538
CACACGT35800.023.37646512
CGAGTGG34550.023.24804733
ACGAGTG34800.023.01649732
CAGTCAC35950.022.78214527
ACACGTC39100.021.28844513
TCTCGTA39400.020.5609743
CACGAGT38950.020.44873231
CCAGTCA42200.019.46171826
Produced by FastQC (version 0.11.5)