FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6149939
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG786691.2791834195428606TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG98550.041.75963645
CGTAATC97700.041.40596838
CTCGTAT98700.041.19496544
ACGTCTG104700.040.22623415
CCGTACG103600.040.04625733
CGTCTGA105950.039.7941116
ACCGTAC104100.039.7458532
TACGTAA103100.039.73726336
ACGTAAT103300.039.68339537
GTAATCT103600.039.13732539
ATCGGAA108400.038.9696432
TCGGAAG109450.038.575233
TAATCTC106050.038.23378440
CACCGTA108200.038.19880331
ACACGTC110500.038.1536713
CACACGT110550.038.0957112
TCTCGTA107000.038.08358843
CACGTCT111100.037.9491614
AATCTCG108850.037.47766541
CAGTCAC111000.037.44005627
Produced by FastQC (version 0.11.5)