FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6658768
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG757251.1372223810771TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG94500.042.19410345
CTCGTAT97050.041.03969244
CGTTTCG102900.040.9515633
AGTCACG104550.040.19978728
TTCGGAA103450.040.0164636
GTTTCGG105750.039.9135334
TTTCGGA105000.039.70429235
ACGTTTC106450.039.6281432
ACGTCTG108450.039.18629515
CGTCTGA108550.039.15019216
GTCACGT107350.039.10875729
TCGGAAT107000.038.5007337
CGGAATC105450.038.42771538
ATCGGAA112300.038.1385082
TCGGAAG112550.038.0337943
CACGTTT111950.037.70134431
AATCTCG108000.037.3996141
TCTCGTA106700.037.30695343
CACACGT113200.037.30294812
GATCGGA115400.037.1217961
Produced by FastQC (version 0.11.5)