FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9947134
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG362250.36417524887067976TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG48600.037.7364345
ACGTCTG54100.034.93092715
CGTCTGA56400.033.46655316
AGTCACG55350.033.3753428
CTCGTAT56250.032.84430744
CACGTGG56350.032.74114631
ATCGGAA59100.031.8330292
TCGGAAG59850.031.546923
GTCACGT58600.031.52400229
GCCTTAT58350.031.000437
TATCTCG59000.030.74131841
CACGTCT61700.030.70071614
GGCCTTA59950.030.39713936
ACGTGGC60350.030.34706732
CACACGT63450.029.53382312
TCACGTG62800.029.3785530
CTTATCT62750.029.01031539
ACACGTC67050.028.35072913
CAGTCAC66600.028.0083227
TCTCGTA67400.027.4776443
Produced by FastQC (version 0.11.5)