FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10658714
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG168650.15822734337369407TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29400.033.44691545
ACGTCTG30050.033.3906415
AGTCACG29500.033.0251728
CGTCTGA30650.031.92957316
CGTGAAA30850.031.21385233
CTCGTAT29700.030.45831144
ACGTGAA31850.030.44574732
AACGATC29900.029.12116438
CACGTGA33800.029.02208331
TGAAACG33450.028.58440835
ATCGGAA35100.028.3408262
GTCACGT34400.028.19005829
GAAACGA34800.027.60469636
TCGGAAG36100.027.5557633
AAACGAT34950.027.10114337
CACGTCT39450.025.4913514
CACACGT39400.025.2942312
TCACGTG39450.024.63743830
CAGTCAC43350.023.14861127
ACACGTC44450.022.77480713
Produced by FastQC (version 0.11.5)