FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12455249
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG153810.12349010445315063TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG26650.031.57186715
AGTCACG27500.030.27327528
CGTCTGA28600.029.96986616
TCGTATG28250.029.3925145
CTCGTAT31550.026.03403344
ACGTCCG31450.025.89757732
GTCACGT33700.024.70351229
TCGGAAG34650.024.680433
ATCGGAA34600.024.586012
CACGTCT34400.024.52422114
GTCCGCA33150.024.43394934
CCGCACA35950.022.46604536
TCACGTC37400.022.2587830
CACACGT38500.021.97054512
CAGTCAC40500.021.00080127
CGCACAT40650.020.03525237
ACACGTC43300.019.84673713
GATCGGA46950.018.4103551
TCCGCAC45200.018.11768735
TCTCGTA47100.017.82115443
Produced by FastQC (version 0.11.5)