FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11722313
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG235560.2009501025949401TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG38900.033.77465415
CGTCTGA39300.033.2591616
TCGTATG37550.032.7812545
CTCGTAT39400.031.29941244
GTCCGCA39750.031.29833236
ATCGGAA46300.027.9472222
CACACGT47050.027.54027712
TCGGAAG47400.027.2037053
CACGTCT48100.027.0805514
CGCATCT46350.026.45996939
ACCCGTC49400.025.86949532
TCCGCAT48500.025.79198837
ACACGTC51750.025.21291713
CAGTCAC51450.025.14699627
TCACCCG51800.024.67090830
TCTCGTA52650.023.59350843
CCGTCCG55550.023.33027334
AGTCACC55400.023.27209928
GATCGGA57400.022.5491471
CCCGTCC57250.022.1257333
Produced by FastQC (version 0.11.5)