FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10644350
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG180460.16953595099747754TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG25850.033.6008845
ACGTCTG30950.033.3646215
CGTCTGA32250.032.0196916
CTCGTAT27350.031.10058844
ATCGGAA36450.028.4020392
TCGGAAG37450.027.6436393
CACGTCT38750.026.76476114
GTCAGAA38650.026.01884836
CACACGT40700.025.31540112
TGTCAGA40700.025.26107635
AATCTCG37950.024.90414641
GTCACAT41600.024.55578429
AGTCACA42850.023.78705428
CAGTCAC43250.023.67110827
ACACGTC45250.023.21731813
ATGTCAG48200.021.70554434
CGGAAGA48900.021.631084
GATCGGA48750.021.2874531
CCAGTCA50200.020.4396226
CACATGT50850.020.35305431
Produced by FastQC (version 0.11.5)