FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11037281
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG469560.42543086472112107TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG58800.038.30744645
CCGTATC60900.036.87063638
TCCGTAT62400.036.3076837
CGTATCT61850.036.1997739
CTCGTAT62450.035.96121244
AGTTCCG63750.035.85773534
TTCCGTA65700.034.55061736
ACGTCTG68400.034.04193515
CGTCTGA69350.033.54316716
TATCTCG66850.033.39231541
GTTCCGT71150.032.0942435
TCGGAAG76050.030.4796313
ATCGGAA76200.030.4786972
CACGTCT77300.030.15131814
TCTCGTA75200.030.10345843
GTCACAG76650.030.05915629
ACAGTTC77700.029.41995632
CACACGT79250.029.32320212
TCACAGT78700.029.24652130
CAGTCAC78800.029.23941427
Produced by FastQC (version 0.11.5)