FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l07_n01_C1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l07_n01_C1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11116642
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG190470.17133771151396257TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29400.033.9061845
ACGTCTG31500.032.13902315
CGTCTGA32950.030.51987616
CTCGTAT31350.029.21374344
ATCGGAA38400.026.371992
TCGGAAG38800.026.1001113
CACGTCT40050.025.61492214
CACACGT40200.025.5183112
CACAGTC40500.024.66627531
ACACGTC43550.024.0202713
GTCACAG41900.023.73545329
CAGTCAC42650.023.42410527
TCACAGT43450.022.73266430
AGTCACA44550.022.475428
AATCTCG41500.021.7975841
ACAGTCA46650.021.55904632
GTCAACA47850.020.59398735
GATCGGA50150.020.1525881
CGGAAGA51600.019.887344
TCTCGTA47350.019.86491243
Produced by FastQC (version 0.11.5)