FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14028660
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT5100.026.0527462
GTAGATC2950.024.40618932
TAGATCT3250.022.84560233
AGTGTAG3200.021.79627229
TCTCGGT3350.021.49140437
GAGTGTA3300.021.13404528
GTGTAGA3700.018.85082830
AACCGCT3500.017.99950231
TGTAGAT3900.017.88411931
ATCGGAA9400.017.7286472
TCGGAAG9250.017.5283373
GATCTCG4800.017.34320835
CGGTTCG4950.017.26910812
TGAACCG3800.017.17057829
AGCGGTT6000.016.87146610
CTCGGTG4450.016.68451338
GGTTCGG4600.016.6269513
GATCGGA10350.015.8837931
AGATCTC5100.015.88185534
TCCCTAC3000.015.7467019
Produced by FastQC (version 0.11.5)