FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26551060
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26700.012.2301792
GTACTCG51950.010.7065774
GTAGAAC33500.010.2067611
GCGTGAC36900.010.192161
AGTACTC59400.09.5538213
GGAAAAT110750.09.4148481
CACTCGT68000.09.3637235
CTCTATA67350.09.3520245
TACTCGA59950.09.1258745
TCACACG27300.09.06369415
GAATCGA40900.08.974364
AAAATCC83000.08.9536233
TCACTCG70800.08.92984634
ACTCGTG70350.08.88999836
GCGGAAA82200.08.8766541
ACACGCG25700.08.5801196
TCAGGTA41550.08.4471587
GAATTGA69150.08.3365624
GTGACTC45050.08.148093
AATCGAA54250.07.88517335
Produced by FastQC (version 0.11.5)