FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25504330
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG37350.012.4808633
ACTTTCG38300.012.27861519
GTAGAAC40500.012.27519611
TAATCGA41000.012.2479834
GCCCTAT50900.010.16485610
GGTAAGT23500.010.05129317
TAGAACA50750.09.92894612
ATAGGGG48500.09.78823234
ATTAGGG44100.09.7450266
GGAAAAT116400.09.5384831
TGCTAAC89900.09.33340844
AATCGAA44650.09.2275845
TGCCCTA57550.09.1857319
TGGTAGG53800.09.15758927
GGTAGAA55750.09.03845710
TCAGGTA59250.09.0372617
GCTAATC52000.08.9650432
AATCGAG62450.08.94082455
TTAGGGT45050.08.9393617
CGTTAAC92100.08.917335521
Produced by FastQC (version 0.11.5)