FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25465250
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC35800.010.11659211
CACTCGT52950.09.26318635
CGTGACT23000.09.0082662
GTACTCG39050.08.8805334
AATCGAA47550.08.71227555
CTCGAAT23800.08.7054661
TCACTCG56650.08.6184634
GCGGAAA65750.08.5972421
ACTCGTG56400.08.57578336
CTCTATA59800.08.38836245
GGAAAAT101600.08.3787521
TAGGCGG36200.07.831279331
GCGTGAC31450.07.80525451
AAAATCC70250.07.7577343
GCATAGG34550.07.74878841
AGTACTC51350.07.6747363
GGTAGAA48400.07.668844710
CGGAAAC69600.07.66867452
GAATTGA62600.07.6620444
TTACGAC18400.07.58131629
Produced by FastQC (version 0.11.5)