FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24385733
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22550.012.1839622
GTACTCG41700.010.3681694
GTAGAAC32550.09.4680211
AATCGAA44550.09.4000645
AGTACTC50250.09.3215143
GCGGAAA62350.09.210411
GCGTGAC32850.09.0492751
CACTCGT55050.08.90972935
GGAAAAT95300.08.8852611
ACTCGTG55950.08.80550136
CTCGAAT20850.08.6409031
CTCTATA57800.08.48427545
TACTCGA50450.08.4792625
GAATCGA37900.08.3775454
TCACTCG59100.08.10881634
CGGCTAT29950.08.045671
TTGCGTC26400.08.01111230
TCACACG24200.07.993829715
TCCGTTA30750.07.829568419
ACTTTCG18700.07.82115519
Produced by FastQC (version 0.11.5)