FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26370442
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCG48050.09.9819144
CGTGACT24250.09.4723392
AATCGAA53450.09.3931885
CTCTATA67000.09.19969145
GCGGAAA77650.08.8456141
ACTCGTG70350.08.825932536
TACTCGA53400.08.8117255
CACTCGT68750.08.73774635
AGTACTC56100.08.5500743
GAATCGA36500.08.5136114
CTCGAAT24000.08.44502451
CGGAAAT24550.08.3475591
AAAATCC76200.08.3338813
ACACGCG26400.08.267346
GGAAAAT102750.08.2628361
GTAGAAC30450.08.20025511
TCACTCG75400.08.14614634
GCGTGAC32400.08.0627391
AAAAATC76050.07.93605042
TCACACG25050.07.812485715
Produced by FastQC (version 0.11.5)