FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29654449
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28900.011.1432782
GTACTCG51400.010.1201854
CTCTATA70550.09.46980645
CTCGAAT27400.09.2053911
ACTCGTG73050.09.17722436
AGTACTC60500.09.0822893
CACTCGT73300.09.05498135
GCGGAAA84400.08.9921231
AATCCGC45600.08.9366385
GCGTGAC41700.08.8569161
GTAGAAC35050.08.60015811
TCACTCG77200.08.59754134
GGAAAAT115200.08.5819671
TACTCGA59700.08.523045
TAAGTCT29900.08.3601223
AGTCTCG27250.08.3447885
AAAATCC88350.08.2584653
GAATTGA71450.08.2257954
CGGAAAT26550.07.88847731
TTAAGTC33850.07.8505142
Produced by FastQC (version 0.11.5)