FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28184624
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25000.012.0713622
GTAGAAC40600.010.52713411
GCGTGAC33900.09.8986971
GTACTCG49650.09.8874954
GGAAAAT121950.09.4738621
CTCGAAT24150.09.1390361
AAAATCC87850.08.9978723
AGTACTC62050.08.6742243
AATCGAA55900.08.6595995
TAGAACA52300.08.3441612
CACTCGT52850.08.34413935
GAATCGA43050.08.21250254
TACTCGA59650.08.1529445
GGTAGAA52850.08.04450710
CTCTATA53700.08.04279945
TCAGGTA56900.08.0268897
AATTCAC49100.07.9329685
GAATTGA68450.07.92852934
TCACTCG55400.07.757004334
TCCGTTA32250.07.604952319
Produced by FastQC (version 0.11.5)