FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30087115
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26500.012.6624112
GTAGAAC38050.011.11467511
GTACTCG62750.010.6579034
AGTACTC69600.09.7066793
GCGTGAC39250.09.6393181
TTTCGGG32350.09.45859837
GGAAAAT126150.09.1045741
ACACGCG29250.09.0003256
AATCGAA57150.08.8245725
ATTTCGG28850.08.65567736
CGGGTAG7950.08.48861515
ACTTTCG35900.08.46130919
GTGACTC44100.08.2724693
AAAATCC93100.08.0789593
TCCGTTA79050.08.05531919
GCGATTT90800.08.0359251
CACTCGT60100.07.973967635
TAGAACA52200.07.929409512
ACATAGC73200.07.897972610
AATCCGC46250.07.88615135
Produced by FastQC (version 0.11.5)