FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n02_B1W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n02_B1W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22896882
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24400.013.7523772
GCGTGAC32200.011.1903951
GTAGAAC29650.010.545876511
GTACTCG45000.09.9082724
GTGACTC37300.09.6600253
TCACACG22700.09.61235615
GGAAAAT93800.09.4116231
AGTACTC51850.09.1643313
AAAATCC72550.08.9707243
TACTCGA48750.08.9595255
ACACGCG23850.08.6793626
CTCGAAT18450.08.66649151
GAATCGA32950.08.3377654
AAATCCA81800.08.0384964
GAATTGA58100.07.984294
AATCGAA41900.07.95253475
GCGGAAA54200.07.89469961
ACTCGTG45900.07.89095336
CGCGATA9200.07.825815729
AGAACGG121800.07.78398283
Produced by FastQC (version 0.11.5)