FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14028660
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA4800.036.9738116
ACGTCTG4950.036.30722815
ACACGTC5800.031.42283613
CACGTCT6000.029.6256114
CACACGT6200.029.39555712
CAGTCAC4700.027.30173327
AGCACAC6900.025.76213810
CCAGTCA5400.023.76423326
ATCGGAA10550.022.1514532
TCGGAAG10800.021.6386873
GCACACG8350.021.55717711
GATCGGA11150.020.963411
CTACGAG550.003967470520.428864
CTCCAGT7550.019.97842624
AACTCCA8700.019.66645822
CGGAAGA11950.019.5574
GAGACCG3500.018.65382831
CGAGACC3900.018.47247130
CCTACAC2700.017.51461442
TACACGA3150.017.15938244
Produced by FastQC (version 0.11.5)