FastQCFastQC Report
Wed 3 Apr 2019
CBKDUACXX_l06_n01_B1W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBKDUACXX_l06_n01_B1W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26551060
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG354470.13350502767121164TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59150.031.45232615
CGTCTGA61300.030.34918416
CGTAATC59050.030.10589638
CCGTACG62950.029.16631133
TCGTATG62750.028.80023845
CTCGTAT63300.028.37238744
CACCGTA68050.026.9802431
ACCGTAC71400.025.87132
TCGGAAG73450.025.457323
ATCGGAA77600.024.1248762
CACGTCT80700.023.27657314
GTAATCT79550.022.74515239
CACACGT85500.021.9961312
CAGTCAC87800.021.50002727
TACGTAA85150.021.24532536
AATCTCG86550.021.19270941
ACGTAAT85500.021.10715537
TAATCTC90500.020.16824740
TCTCGTA90500.020.11873843
AGTCACC99000.019.29522128
Produced by FastQC (version 0.11.5)